› 4gou

X-ray diffraction
2.3Å resolution

Crystal structure of an RGS-RhoGEF from Entamoeba histolytica

Released:
DOI: 10.2210/pdb4gou/pdb
PDB entry 4gou coloured by chain, front view.
PDB entry 4gou coloured by chain, side view.
PDB entry 4gou coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis.
Bosch DE, Kimple AJ, Manning AJ, Muller RE, Willard FS, Machius M, Rogers SL, Siderovski DP
Structure 21 65-75 (2013)
PMID: 23260656

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of g protein signaling domain containing protein Chain: A
Molecule details ›
Chain: A
Length: 518 amino acids
Theoretical weight: 60.45 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4LYV4 (Residues: 2-517; Coverage: 99%)
Gene name: EHI_010670
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 86.124Å b: 46.259Å c: 142.637Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.185 0.182 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Regulation of virulence of Entamoeba histolytica.
Marie et al. (2014)
1 more

4 mentions without citation

Interfacial inhibitors.
Pommier et al. (2015)
3 more