› 4gra

X-ray diffraction
2.56Å resolution

Crystal structure of SULT1A1 bound with PAP

Released:
DOI: 10.2210/pdb4gra/pdb
PDB entry 4gra coloured by chain, front view.
PDB entry 4gra coloured by chain, side view.
PDB entry 4gra coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The gate that governs sulfotransferase selectivity.
Cook I, Wang T, Almo SC, Kim J, Falany CN, Leyh TS
Biochemistry 52 415-24 (2013)
PMID: 23256751

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 34.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50225 (Residues: 1-295; Coverage: 100%)
Gene names: OK/SW-cl.88, STP, STP1, SULT1A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 48.296Å b: 122.428Å c: 55.021Å
α: 90° β: 92.12° γ: 90°
R-values:
R R work R free
0.216 0.214 0.266
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The Regulation of Steroid Action by Sulfation and Desulfation.
Mueller et al. (2015)
9 more

8 mentions without citation

From Steroid and Drug Metabolism to Glycobiology, Using Sulfotransferase Structures to Understand and Tailor Function.
Pedersen et al. (2022)
7 more