› 4grd

X-ray diffraction
1.85Å resolution

Crystal structure of Phosphoribosylaminoimidazole carboxylase catalytic subunit from Burkholderia cenocepacia J2315

Released:
DOI: 10.2210/pdb4grd/pdb
PDB entry 4grd coloured by chain, front view.
PDB entry 4grd coloured by chain, side view.
PDB entry 4grd coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide mutase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 173 amino acids
Theoretical weight: 18.15 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4EA21 (Residues: 1-173; Coverage: 100%)
Gene names: BCAL2837, purE
Sequence domains: AIR carboxylase
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I222
Unit cell:
a: 116.51Å b: 117.18Å c: 122.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.176
Expression system: Escherichia coli BL21(DE3)

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