› 4grs

X-ray diffraction
3Å resolution

Crystal structure of a chimeric DAH7PS

Released:
DOI: 10.2210/pdb4grs/pdb
PDB entry 4grs coloured by chain, front view.
PDB entry 4grs coloured by chain, side view.
PDB entry 4grs coloured by chain, top view.
Source organisms:
Primary publication:
Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain.
Cross PJ, Allison TM, Dobson RC, Jameson GB, Parker EJ
Proc Natl Acad Sci U S A 110 2111-6 (2013)
PMID: 23345433

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DAHP synthetase I/KDSA domain-containing protein; Phospho-2-dehydro-3-deoxyheptonate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 333 amino acids
Theoretical weight: 37.11 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYH8 (Residues: 1-103; Coverage: 31%)
  • Canonical: Q8U0A9 (Residues: 33-262; Coverage: 88%)
Gene names: PF1690, TM_0343, aroF
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 4 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 76.901Å b: 130.852Å c: 138.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.248
Expression system: Escherichia coli

Quick links

Citations

7 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
6 more

3 mentions without citation

X-ray Scattering Studies of Protein Structural Dynamics.
Meisburger et al. (2017)
2 more