› 4grv

X-ray diffraction
2.8Å resolution

The crystal structure of the neurotensin receptor NTS1 in complex with neurotensin (8-13)

Released:
DOI: 10.2210/pdb4grv/pdb
PDB entry 4grv coloured by chain, front view.
PDB entry 4grv coloured by chain, side view.
PDB entry 4grv coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the agonist-bound neurotensin receptor.
White JF, Noinaj N, Shibata Y, Love J, Kloss B, Xu F, Gvozdenovic-Jeremic J, Shah P, Shiloach J, Tate CG, Grisshammer R
Nature 490 508-13 (2012)
PMID: 23051748

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132965 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endolysin; Neurotensin receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 57.2 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P20789 (Residues: 43-268, 300-396; Coverage: 76%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, Ntsr, Ntsr1
Sequence domains:
Structure domains:
Neurotensin Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 819 Da
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P20068 (Residues: 157-162; Coverage: 4%)
Gene name: Nts

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 49.96Å b: 69.619Å c: 97.55Å
α: 90° β: 101.75° γ: 90°
R-values:
R R work R free
0.228 0.225 0.282
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

71 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
70 more

77 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
76 more