› 4gt7

X-ray diffraction
2.61Å resolution

An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation

Released:
DOI: 10.2210/pdb4gt7/pdb
PDB entry 4gt7 coloured by chain, front view.
PDB entry 4gt7 coloured by chain, side view.
PDB entry 4gt7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, nonreceptor binding conformation.
Wurzburg BA, Kim B, Tarchevskaya SS, Eggel A, Vogel M, Jardetzky TS
J Biol Chem 287 36251-7 (2012)
PMID: 22948141

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Immunoglobulin heavy constant epsilon Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 221 amino acids
Theoretical weight: 24.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01854 (Residues: 210-426; Coverage: 40%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 106.753Å b: 104.807Å c: 45.899Å
α: 90° β: 94.51° γ: 90°
R-values:
R R work R free
0.192 0.19 0.253
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Development of small molecules to target the IgE:FcεRI protein-protein interaction in allergies.
Smith et al. (2013)
5 other articles

4 mentions without citation

Structural and Physical Basis for Anti-IgE Therapy.
Wright et al. (2015)
3 more