› 4gu3

X-ray diffraction
3.6Å resolution

Crystal structure of the T1L reovirus attachment protein sigma1 in complex with the GM2 glycan

Released:
DOI: 10.2210/pdb4gu3/pdb
PDB entry 4gu3 coloured by chain, front view.
PDB entry 4gu3 coloured by chain, side view.
PDB entry 4gu3 coloured by chain, top view.
Source organism: Mammalian orthoreovirus 1 Lang
Primary publication:
The GM2 glycan serves as a functional coreceptor for serotype 1 reovirus.
Reiss K, Stencel JE, Liu Y, Blaum BS, Reiter DM, Feizi T, Dermody TS, Stehle T
PLoS Pathog 8 e1003078 (2012)
PMID: 23236285

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Outer capsid protein sigma-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 219 amino acids
Theoretical weight: 24.5 KDa
Source organism: Mammalian orthoreovirus 1 Lang
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04506 (Residues: 261-470; Coverage: 45%)
Gene name: S1
Sequence domains: Reovirus viral attachment protein sigma 1
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, SIA, NGA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 147.46Å b: 147.46Å c: 164.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.204
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

Virus-Receptor Interactions: The Key to Cellular Invasion.
Maginnis MS. (2018)
12 more

8 mentions without citation

The sweet spot: defining virus-sialic acid interactions.
Stencel-Baerenwald et al. (2014)
7 more