› 4gvl

X-ray diffraction
3Å resolution

Crystal Structure of the GsuK RCK domain

Released:
DOI: 10.2210/pdb4gvl/pdb
PDB entry 4gvl coloured by chain, front view.
PDB entry 4gvl coloured by chain, side view.
PDB entry 4gvl coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Primary publication:
Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.
Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y
Elife 1 e00184 (2012)
PMID: 23240087

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-181064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TrkA domain protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 462 amino acids
Theoretical weight: 50.14 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q74FS9 (Residues: 107-564; Coverage: 81%)
Gene name: GSU0527
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand AMP 4 x AMP
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 110.648Å b: 161.693Å c: 310.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

BK channels: multiple sensors, one activation gate.
Yang et al. (2015)
6 more

1 mention without citation

Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.
Kong et al. (2012)