› 4gwj

X-ray diffraction
1.6Å resolution

His 62 mutant of the lectin binding domain of Lectinolysin complexed with Lewis b

Released:
DOI: 10.2210/pdb4gwj/pdb
PDB entry 4gwj coloured by chain, front view.
PDB entry 4gwj coloured by chain, side view.
PDB entry 4gwj coloured by chain, top view.
Source organism: Streptococcus mitis
Primary publication:
Manipulating the Lewis antigen specificity of the cholesterol-dependent cytolysin lectinolysin.
Lawrence SL, Feil SC, Holien JK, Kuiper MJ, Doughty L, Dolezal O, Mulhern TD, Tweten RK, Parker MW
Front Immunol 3 330 (2012)
PMID: 23181061

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Thiol-activated cytolysin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.84 KDa
Source organism: Streptococcus mitis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PHL4 (Residues: 38-190; Coverage: 24%)
Gene names: SMNM65_03180, samhpaf, sm-hpaf
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, FUC
Carbohydrate polymer
2 bound ligands:
Ligand MG 3 x MG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 66.923Å b: 66.923Å c: 99.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.21
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural biology of antibody recognition of carbohydrate epitopes and potential uses for targeted cancer immunotherapies.
Dingjan et al. (2015)
1 more

1 mention without citation

Manipulating the Lewis antigen specificity of the cholesterol-dependent cytolysin lectinolysin.
Lawrence et al. (2012)