› 4gwm

X-ray diffraction
1.85Å resolution

Crystal structure of human promeprin beta

Released:
DOI: 10.2210/pdb4gwm/pdb
PDB entry 4gwm coloured by chain, front view.
PDB entry 4gwm coloured by chain, side view.
PDB entry 4gwm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the sheddase function of human meprin β metalloproteinase at the plasma membrane.
Arolas JL, Broder C, Jefferson T, Guevara T, Sterchi EE, Bode W, Stöcker W, Becker-Pauly C, Gomis-Rüth FX
Proc Natl Acad Sci U S A 109 16131-6 (2012)
PMID: 22988105

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His(5)-|-Leu-, -Leu(6)-|-Cys-, -Ala(14)-|-Leu- and -Cys(19)-|-Gly- bonds in insulin B chain.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (8 distinct):
Meprin A subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 592 amino acids
Theoretical weight: 67.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q16820 (Residues: 23-614; Coverage: 87%)
Gene name: MEP1B
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
5 bound ligands:
Ligand ZN 2 x ZN
Ligand NA 2 x NA
Ligand NAG 2 x NAG
Ligand CL 5 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 69.62Å b: 71.12Å c: 85.74Å
α: 74.87° β: 80.08° γ: 65.13°
R-values:
R R work R free
0.168 0.168 0.188
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

Meprin α and meprin β: Procollagen proteinases in health and disease.
Prox et al. (2015)
7 more

20 mentions without citation

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
19 more