› 4gx8

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit alpha; DNA polymerase III subunit epsilon Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 315 amino acids
Theoretical weight: 34.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03007 (Residues: 208-243; Coverage: 15%)
  • Canonical: P10443 (Residues: 1-270; Coverage: 23%)
Gene names: JW0179, JW0205, b0184, b0215, dnaE, dnaQ, mutD, polC
Sequence domains: PHP domain
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 84.47Å b: 56.63Å c: 138.01Å
α: 90° β: 93.52° γ: 90°
R-values:
R R work R free
0.214 0.213 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

DNA Polymerases Divide the Labor of Genome Replication.
Lujan et al. (2016)
1 more

3 mentions without citation

Novel Antibiotics Targeting Bacterial Replicative DNA Polymerases.
Santos et al. (2020)
2 more