› 4gx9

X-ray diffraction
2.15Å resolution

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit alpha; DNA polymerase III subunit epsilon Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 324 amino acids
Theoretical weight: 35.7 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03007 (Residues: 200-243; Coverage: 18%)
  • Canonical: P10443 (Residues: 1-270; Coverage: 23%)
Gene names: JW0179, JW0205, b0184, b0215, dnaE, dnaQ, mutD, polC
Sequence domains: PHP domain
Structure domains: Metal-dependent hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 83.017Å b: 56.98Å c: 135.073Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.229 0.226 0.291
Expression system: Escherichia coli

Quick links

Citations

3 review citations

DNA Polymerases Divide the Labor of Genome Replication.
Lujan et al. (2016)
2 more

2 mentions without citation

Comprehensive analysis of DNA polymerase III α subunits and their homologs in bacterial genomes.
Timinskas et al. (2014)
1 more