› 4gxo

X-ray diffraction
2.05Å resolution

Crystal structure of Staphylococcus aureus protein SarZ mutant C13E

Released:
DOI: 10.2210/pdb4gxo/pdb
PDB entry 4gxo coloured by chain, front view.
PDB entry 4gxo coloured by chain, side view.
PDB entry 4gxo coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Primary publication:
Protein cysteine phosphorylation of SarA/MgrA family transcriptional regulators mediates bacterial virulence and antibiotic resistance.
Sun F, Ding Y, Ji Q, Liang Z, Deng X, Wong CC, Yi C, Zhang L, Xie S, Alvarez S, Hicks LM, Luo C, Jiang H, Lan L, He C
Proc Natl Acad Sci U S A 109 15461-6 (2012)
PMID: 22927394

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102047 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MarR family regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 16.44 KDa
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3KA72 (Residues: 7-142; Coverage: 92%)
Gene name: NWMN_2286
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 45.442Å b: 55.792Å c: 119.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Regulation of Staphylococcus aureus Virulence.
Jenul et al. (2019)
29 more