› 4gzj

X-ray diffraction
1.55Å resolution

Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose and laminaratetrose

Released:
DOI: 10.2210/pdb4gzj/pdb
PDB entry 4gzj coloured by chain, front view.
PDB entry 4gzj coloured by chain, side view.
PDB entry 4gzj coloured by chain, top view.
Source organism: Solanum tuberosum
Primary publication:
Structures of an active-site mutant of a plant 1,3-β-glucanase in complex with oligosaccharide products of hydrolysis.
Wojtkowiak A, Witek K, Hennig J, Jaskolski M
Acta Crystallogr D Biol Crystallogr 69 52-62 (2013)
PMID: 23275163

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
1,3-beta-D-glucan glucanohydrolase (Glucan endo-1,3-beta-glucosidase a) Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.55 KDa
Source organism: Solanum tuberosum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q70C53 (Residues: 24-338; Coverage: 100%)
Gene name: gluB20-2
Sequence domains: Glycosyl hydrolases family 17
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 55.307Å b: 49.202Å c: 57.431Å
α: 90° β: 98.84° γ: 90°
R-values:
R R work R free
0.152 0.151 0.181
Expression system: Escherichia coli BL21

Quick links

Citations

7 citation in other articles

A phylogenetic approach to study the origin and evolution of plasmodesmata-localized glycosyl hydrolases family 17.
Gaudioso-Pedraza et al. (2014)
6 more

2 mentions without citation

Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides.
Déjean et al. (2020)
1 more