› 4h0o

X-ray diffraction
2.4Å resolution

Crystal Structure of Acetate Kinase from Entamoeba histolytica

Released:
DOI: 10.2210/pdb4h0o/pdb
PDB entry 4h0o coloured by chain, front view.
PDB entry 4h0o coloured by chain, side view.
PDB entry 4h0o coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans.
Thaker TM, Tanabe M, Fowler ML, Preininger AM, Ingram-Smith C, Smith KS, Iverson TM
J Struct Biol 181 185-9 (2013)
PMID: 23159802

Function and Biology Details

Reaction catalysed: 
ATP + acetate = ADP + acetyl phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-111325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable acetate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 44.7 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4M1C3 (Residues: 1-392; Coverage: 100%)
Gene name: EHI_170010
Sequence domains: Acetokinase family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I222
Unit cell:
a: 98.885Å b: 126.874Å c: 145.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Virulence-Associated Enzymes of Cryptococcus neoformans.
Almeida et al. (2015)
1 more

2 mentions without citation

Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans.
Thaker et al. (2013)
1 more