› 4h1g

X-ray diffraction
2.15Å resolution

Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein

Released:
DOI: 10.2210/pdb4h1g/pdb
PDB entry 4h1g coloured by chain, front view.
PDB entry 4h1g coloured by chain, side view.
PDB entry 4h1g coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the Candida albicans Kar3 kinesin motor domain fused to maltose-binding protein.
Delorme C, Joshi M, Allingham JS
Biochem Biophys Res Commun 428 427-32 (2012)
PMID: 23137538

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Kinesin-like protein; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 715 amino acids
Theoretical weight: 79.08 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384, 386-387; Coverage: 97%)
  • Canonical: C4YKK8 (Residues: 346-346, 352-694; Coverage: 50%)
Gene names: CAWG_06014, JW3994, b4034, malE
Sequence domains:
Structure domains: Kinesin motor domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 49.463Å b: 74.927Å c: 98.673Å
α: 90° β: 96.44° γ: 90°
R-values:
R R work R free
0.171 0.169 0.211
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein.
Park et al. (2016)
1 more

2 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
1 more