› 4h1i

X-ray diffraction
3.1Å resolution

Structure of human thymidylate synthase at low salt conditions

Released:
DOI: 10.2210/pdb4h1i/pdb
PDB entry 4h1i coloured by chain, front view.
PDB entry 4h1i coloured by chain, side view.
PDB entry 4h1i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Analysis of mRNA recognition by human thymidylate synthase.
Brunn ND, Dibrov SM, Kao MB, Ghassemian M, Hermann T
Biosci Rep 34 e00168 (2014)
PMID: 25423174

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 318 amino acids
Theoretical weight: 36.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04818 (Residues: 1-313; Coverage: 100%)
Gene names: OK/SW-cl.29, TS, TYMS
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 157.855Å b: 94.927Å c: 131.439Å
α: 90° β: 122.75° γ: 90°
R-values:
R R work R free
0.234 0.232 0.285
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Detection of Genomic Uracil Patterns.
Békési et al. (2021)
1 more

2 mentions without citation

Analysis of mRNA recognition by human thymidylate synthase.
Brunn et al. (2014)
1 more