4h1s

X-ray diffraction
2.2Å resolution

Crystal Structure of a Truncated Soluble form of Human CD73 with Ecto-5'-Nucleotidase activity

Released:

Function and Biology Details

Reactions catalysed:
Thymidylate + H(2)O = thymidine + phosphate
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate
CMP + H(2)O = cytidine + phosphate
IMP + H(2)O = inosine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-nucleotidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 530 amino acids
Theoretical weight: 59.04 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P21589 (Residues: 27-549; Coverage: 95%)
Gene names: NT5, NT5E, NTE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Unit cell:
a: 54.91Å b: 95.01Å c: 230.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.217