› 4h2c

X-ray diffraction
1.7Å resolution

Trehalulose synthase MutB R284C mutant

Released:
DOI: 10.2210/pdb4h2c/pdb
PDB entry 4h2c coloured by chain, front view.
PDB entry 4h2c coloured by chain, side view.
PDB entry 4h2c coloured by chain, top view.
Source organism: Rhizobium sp. MX-45
Primary publication:
Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity.
Lipski A, Watzlawick H, Ravaud S, Robert X, Rhimi M, Haser R, Mattes R, Aghajari N
Acta Crystallogr D Biol Crystallogr 69 298-307 (2013)
PMID: 23385465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 63.88 KDa
Source organism: Rhizobium sp. MX-45
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1F6 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 84.09Å b: 90.08Å c: 91.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.173
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase.
Mu et al. (2014)
1 more

3 mentions without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
2 more