› 4h42

X-ray diffraction
2.01Å resolution

Synthesis of a Weak Basic uPA Inhibitor and Crystal Structure of Complex with uPA

Released:
DOI: 10.2210/pdb4h42/pdb
PDB entry 4h42 coloured by chain, front view.
PDB entry 4h42 coloured by chain, side view.
PDB entry 4h42 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Synthesis of a Weak Basic uPA Inhibitor and Crystal Structure of Complex with uPA
Yu H-Y, Gao D, Xu Z, Jiang L-G, Hong Z-B, Fan X, Yuan C, Wang J-D, Huang M
Chin. J. Struct. Chem. 32 961-968 (2013)

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 248 amino acids
Theoretical weight: 27.97 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-426; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand 11E 1 x 11E
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R3
Unit cell:
a: 120.916Å b: 120.916Å c: 42.875Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.207 0.285
Expression system: Komagataella pastoris

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