› 4h4g

X-ray diffraction
2.65Å resolution

Crystal Structure of (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase from Burkholderia thailandensis E264

Released:
DOI: 10.2210/pdb4h4g/pdb
PDB entry 4h4g coloured by chain, front view.
PDB entry 4h4g coloured by chain, side view.
PDB entry 4h4g coloured by chain, top view.
Source organism: Burkholderia thailandensis E264
Primary publication:
Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC
PLoS One 8 e53851 (2013)
PMID: 23382856

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 160 amino acids
Theoretical weight: 18.11 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2SWY7 (Residues: 1-160; Coverage: 100%)
Gene names: BTH_I2038, fabZ
Sequence domains: FabA-like domain
Structure domains: Hotdog Thioesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6122
Unit cell:
a: 136.37Å b: 136.37Å c: 364.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.247
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
10 more

1 mention without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)