4h4k

X-ray diffraction
2.8Å resolution

Structure of the Cmr2-Cmr3 subcomplex of the Cmr RNA-silencing complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186419 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CRISPR system Cmr subunit Cmr3 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 36.37 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U1S7 (Residues: 1-322; Coverage: 100%)
Gene names: PF1128, cmr3
Sequence domains: CRISPR-associated protein (Cas_Cmr3)
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I222
Unit cell:
a: 102.523Å b: 135.958Å c: 189.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.214 0.284
Expression system: Escherichia coli BL21(DE3)