› 4h56

X-ray diffraction
3.9Å resolution

Crystal structure of the Clostridium perfringens NetB toxin in the membrane inserted form

Released:
DOI: 10.2210/pdb4h56/pdb
PDB entry 4h56 coloured by chain, front view.
PDB entry 4h56 coloured by chain, side view.
PDB entry 4h56 coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Molecular architecture and functional analysis of NetB, a pore-forming toxin from Clostridium perfringens.
Savva CG, Fernandes da Costa SP, Bokori-Brown M, Naylor CE, Cole AR, Moss DS, Titball RW, Basak AK
J Biol Chem 288 3512-22 (2013)
PMID: 23239883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leukocidin/Hemolysin toxin domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 296 amino acids
Theoretical weight: 33.51 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8ULG6 (Residues: 31-322; Coverage: 100%)
Gene name: netB
Sequence domains: Leukocidin/Hemolysin toxin family
Structure domains: Leukocidin/porin MspA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 313.23Å b: 168.04Å c: 160.46Å
α: 90° β: 109.42° γ: 90°
R-values:
R R work R free
0.285 0.284 0.309
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
13 more

4 mentions without citation

Life at the border: adaptation of proteins to anisotropic membrane environment.
Pogozheva et al. (2014)
3 more