› 4h79

X-ray diffraction
1.9Å resolution

Crystal structure of CasB from Thermobifida fusca

Released:
DOI: 10.2210/pdb4h79/pdb
PDB entry 4h79 coloured by chain, front view.
PDB entry 4h79 coloured by chain, side view.
PDB entry 4h79 coloured by chain, top view.
Source organism: Thermobifida fusca YX
Primary publication:
Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures.
Nam KH, Huang Q, Ke A
FEBS Lett 586 3956-61 (2012)
PMID: 23079036

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated protein, Cse2 family Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.68 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PI5 (Residues: 1-214; Coverage: 100%)
Gene name: Tfu_1599
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
Structure domains: CRISPR-associated protein Cse2

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P61
Unit cell:
a: 86.749Å b: 86.749Å c: 70.016Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.148 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Adaptation in CRISPR-Cas Systems.
Sternberg et al. (2016)
4 more

4 mentions without citation

Structure Principles of CRISPR-Cas Surveillance and Effector Complexes.
Tsui et al. (2015)
3 more