› 4h7a

X-ray diffraction
2.6Å resolution

Crystal structure of CasB from Thermus thermophilus

Released:
DOI: 10.2210/pdb4h7a/pdb
PDB entry 4h7a coloured by chain, front view.
PDB entry 4h7a coloured by chain, side view.
PDB entry 4h7a coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures.
Nam KH, Huang Q, Ke A
FEBS Lett 586 3956-61 (2012)
PMID: 23079036

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated protein Cse2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 19.42 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53VY0 (Residues: 1-156; Coverage: 92%)
Gene names: TTHB189, cse2
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
Structure domains: CRISPR-associated protein Cse2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43212
Unit cell:
a: 100.777Å b: 100.777Å c: 84.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.23
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Adaptation in CRISPR-Cas Systems.
Sternberg et al. (2016)
4 more

2 mentions without citation

Structure Principles of CRISPR-Cas Surveillance and Effector Complexes.
Tsui et al. (2015)
1 more