› 4h7k

X-ray diffraction
1.75Å resolution

Crystal structure of haloalkane dehalogenase LinB I253M mutant from Sphingobium sp. MI1205

Released:
DOI: 10.2210/pdb4h7k/pdb
PDB entry 4h7k coloured by chain, front view.
PDB entry 4h7k coloured by chain, side view.
PDB entry 4h7k coloured by chain, top view.
Source organism: Sphingobium sp. MI1205
Primary publication:
Crystal structure and site-directed mutagenesis analyses of haloalkane dehalogenase LinB from Sphingobium sp. strain MI1205.
Okai M, Ohtsuka J, Imai LF, Mase T, Moriuchi R, Tsuda M, Nagata K, Nagata Y, Tanokura M
J Bacteriol 195 2642-51 (2013)
PMID: 23564170

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.11 KDa
Source organism: Sphingobium sp. MI1205
Expression system: Escherichia coli
UniProt:
  • Canonical: A4PEU6 (Residues: 1-296; Coverage: 100%)
Gene names: K663_22528, K663_22543, dhaA, linB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 50.538Å b: 72.153Å c: 73.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.205
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Dehalogenases: From Improved Performance to Potential Microbial Dehalogenation Applications.
Ang et al. (2018)
1 more

1 mention without citation

Crystal structure and site-directed mutagenesis analyses of haloalkane dehalogenase LinB from Sphingobium sp. strain MI1205.
Okai et al. (2013)