› 4h83

X-ray diffraction
2.09Å resolution

Crystal structure of Mandelate racemase/muconate lactonizing enzyme (EFI target:502127)

Released:
DOI: 10.2210/pdb4h83/pdb
PDB entry 4h83 coloured by chain, front view.
PDB entry 4h83 coloured by chain, side view.
PDB entry 4h83 coloured by chain, top view.
Source organism: marine actinobacterium PHSC20C1
Entry authors: Kim J, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Stead M, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-107257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 388 amino acids
Theoretical weight: 43.49 KDa
Source organism: marine actinobacterium PHSC20C1
Expression system: Escherichia coli
UniProt:
  • Canonical: A4AFX2 (Residues: 1-388; Coverage: 100%)
Gene name: A20C1_08358
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 8 x NA
Ligand BCT 2 x BCT
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 89.983Å b: 102.95Å c: 234.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.147 0.204
Expression system: Escherichia coli

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