› 4h8p

X-ray diffraction
2.05Å resolution

NEAT5 domain of IsdX2, a B. anthracis hemophore in complex with heme

Released:
DOI: 10.2210/pdb4h8p/pdb
PDB entry 4h8p coloured by chain, front view.
PDB entry 4h8p coloured by chain, side view.
PDB entry 4h8p coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
The near-iron transporter (NEAT) domains of the anthrax hemophore IsdX2 require a critical glutamine to extract heme from methemoglobin.
Honsa ES, Owens CP, Goulding CW, Maresso AW
J Biol Chem 288 8479-8490 (2013)
PMID: 23364793

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NEAT domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.71 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8PXZ2 (Residues: 675-797; Coverage: 14%)
Gene name: GBAA_4787
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: I23
Unit cell:
a: 109.02Å b: 109.02Å c: 109.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.223
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Heme Synthesis and Acquisition in Bacterial Pathogens.
Choby et al. (2016)
3 more

3 mentions without citation

The near-iron transporter (NEAT) domains of the anthrax hemophore IsdX2 require a critical glutamine to extract heme from methemoglobin.
Honsa et al. (2013)
2 more