› 4ha4

X-ray diffraction
1.37Å resolution

Structure of beta-glycosidase from Acidilobus saccharovorans in complex with glycerol

Released:
DOI: 10.2210/pdb4ha4/pdb
PDB entry 4ha4 coloured by chain, front view.
PDB entry 4ha4 coloured by chain, side view.
PDB entry 4ha4 coloured by chain, top view.
Source organism: Acidilobus saccharovorans 345-15
Primary publication:
Structures of β-glycosidase from acidilobus saccharovorans in complexes with tris and glycerol.
Trofimov AA, Polyakov KM, Tikhonov AV, Bezsudnova EY, Dorovatovskii PV, Gumerov VM, Ravin NV, Skryabin KG, Popov VO
Dokl Biochem Biophys 449 99-101
PMID: 23657657

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 55.46 KDa
Source organism: Acidilobus saccharovorans 345-15
Expression system: Escherichia coli
UniProt:
  • Canonical: D9PZ08 (Residues: 2-490; Coverage: 100%)
Gene name: ASAC_1390
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

5 bound ligands:
Ligand NA 1 x NA
Ligand SIN 1 x SIN
Ligand GOL 3 x GOL
Ligand PG4 7 x PG4
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: P42212
Unit cell:
a: 84.12Å b: 84.12Å c: 166.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.13 0.163
Expression system: Escherichia coli

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Citations

3 citation in other articles

Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis.
Tiwari et al. (2017)
2 more