› 4haa

X-ray diffraction
1.9Å resolution

Structure of Ribonuclease Binase Glu43Ala/Phe81Ala Mutant

Released:
DOI: 10.2210/pdb4haa/pdb
PDB entry 4haa coloured by chain, front view.
PDB entry 4haa coloured by chain, side view.
PDB entry 4haa coloured by chain, top view.
Source organism: Bacillus intermedius
Primary publication:
Structure and functional studies of the ribonuclease binase Glu43Ala/Phe81Ala mutant.
Mitkevich VA, Schulga AA, Trofimov AA, Dorovatovskii PV, Goncharuk DA, Tkach EN, Makarov AA, Polyakov KM
Acta Crystallogr D Biol Crystallogr 69 991-6 (2013)
PMID: 23695243

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 109 amino acids
Theoretical weight: 12.09 KDa
Source organism: Bacillus intermedius
Expression system: Escherichia coli
UniProt:
  • Canonical: P00649 (Residues: 54-162; Coverage: 82%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: P43
Unit cell:
a: 50.36Å b: 50.36Å c: 196.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.205
Expression system: Escherichia coli

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Citations

1 review citation

Ribonucleases as antiviral agents.
Ilinskaya et al. (2014)
4 other articles