› 4hb5

X-ray diffraction
2.29Å resolution

Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR267.

Released:
DOI: 10.2210/pdb4hb5/pdb
PDB entry 4hb5 coloured by chain, front view.
PDB entry 4hb5 coloured by chain, side view.
PDB entry 4hb5 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Computational design of self-assembling cyclic protein homo-oligomers.
Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D
Nat Chem 9 353-360 (2017)
PMID: 28338692

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Engineered Protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.48 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 56.977Å b: 45.469Å c: 63.152Å
α: 90° β: 107.17° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
9 more

5 mentions without citation

A general computational approach for repeat protein design.
Parmeggiani et al. (2015)
4 more