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› 4hbt

X-ray diffraction
1.1Å resolution

Crystal structure of native CTX-M-15 extended-spectrum beta-lactamase

Released:
DOI: 10.2210/pdb4hbt/pdb
Model geometry
Fit model/data
PDB entry 4hbt coloured by chain, front view.
PDB entry 4hbt coloured by chain, side view.
PDB entry 4hbt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural insight into potent broad-spectrum inhibition with reversible recyclization mechanism: avibactam in complex with CTX-M-15 and Pseudomonas aeruginosa AmpC β-lactamases.
Lahiri SD, Mangani S, Durand-Reville T, Benvenuti M, De Luca F, Sanyal G, Docquier JD
Antimicrob Agents Chemother 57 2496-505 (2013)
PMID: 23439634

Function and Biology Details

Reaction catalysed: 
a beta-lactam + H2O = a substituted beta-amino acid.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EXV5 (Residues: 29-291; Coverage: 100%)
Gene names: AWP47_17965, BANRA_05422, CTX-M-15, F9B07_28185, G5603_26845, HL601_21620, IH772_23850, OGM49_10600, OGM49_26340, PECOH45_0113, PWL68_004872, PWL68_005313, Q2V20_26760, Q2V64_18610, R8O40_005410, R8O40_005528, bla, bla CTX-M-15, blaCTX-M-15, blaCTX-M15, blaUOE-1
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand EDO 8 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 44.698Å b: 45.72Å c: 117.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.117 0.136
Expression system: Escherichia coli

Quick links

Citations

31 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
30 more

48 mentions without citation

Active site comparisons and catalytic mechanisms of the hot dog superfamily.
Labonte et al. (2013)
47 more