› 4hcb

X-ray diffraction
2Å resolution

The metal-free form of crystal structure of E.coli ExoI-ssDNA complex

Released:
DOI: 10.2210/pdb4hcb/pdb
PDB entry 4hcb coloured by chain, front view.
PDB entry 4hcb coloured by chain, side view.
PDB entry 4hcb coloured by chain, top view.
Source organism: Escherichia coli DH5[alpha]
Entry authors: Qiu R, Wei J, Lou T, Liu M, Ji C, Gong W

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117581 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Exodeoxyribonuclease I Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 55.38 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04995 (Residues: 1-475; Coverage: 100%)
Gene names: JW1993, b2011, cpeA, sbcB, xonA
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 62.544Å b: 107.289Å c: 157.574Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.224
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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