› 4hd5

X-ray diffraction
1.9Å resolution

Crystal Structure of BC0361, a polysaccharide deacetylase from Bacillus cereus

Released:
DOI: 10.2210/pdb4hd5/pdb
PDB entry 4hd5 coloured by chain, front view.
PDB entry 4hd5 coloured by chain, side view.
PDB entry 4hd5 coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Structure determination through homology modelling and torsion-angle simulated annealing: application to a polysaccharide deacetylase from Bacillus cereus.
Fadouloglou VE, Kapanidou M, Agiomirgianaki A, Arnaouteli S, Bouriotis V, Glykos NM, Kokkinidis M
Acta Crystallogr D Biol Crystallogr 69 276-83 (2013)
PMID: 23385463

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NodB homology domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 40.87 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81IM3 (Residues: 1-360; Coverage: 100%)
Gene name: BC_0361
Sequence domains: Polysaccharide deacetylase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand ACT 1 x ACT
1 modified residue:
Ligand PXU 1 x PXU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 36.455Å b: 52.76Å c: 93.638Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.18 0.178 0.203
Expression system: Escherichia coli

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Citations

3 citation in other articles

Interrupting peptidoglycan deacetylation during Bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts.
Lambert et al. (2016)
2 more

6 mentions without citation

Substrate Recognition and Specificity of Chitin Deacetylases and Related Family 4 Carbohydrate Esterases.
Aragunde et al. (2018)
5 more