› 4hd7

X-ray diffraction
2.1Å resolution

Crystal Structure of Tyrosinase from Bacillus megaterium V218G mutant soaked in CuSO4

Released:
DOI: 10.2210/pdb4hd7/pdb
PDB entry 4hd7 coloured by chain, front view.
PDB entry 4hd7 coloured by chain, side view.
PDB entry 4hd7 coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Influencing the monophenolase/diphenolase activity ratio in tyrosinase.
Goldfeder M, Kanteev M, Adir N, Fishman A
Biochim Biophys Acta 1834 629-33 (2013)
PMID: 23305929

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosinase copper-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 35.21 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2ZB02 (Residues: 1-297; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 47.9Å b: 78.67Å c: 85.77Å
α: 90° β: 105.91° γ: 90°
R-values:
R R work R free
0.257 0.254 0.282
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Paving the way towards effective plant-based inhibitors of hyaluronidase and tyrosinase: a critical review on a structure-activity relationship.
Gębalski et al. (2022)
3 more

2 mentions without citation

Low- and room-temperature X-ray structures of protein kinase A ternary complexes shed new light on its activity.
Kovalevsky et al. (2012)
1 more