› 4hdq

X-ray diffraction
1.95Å resolution

Crystal Structure of the Ternary Complex of KRIT1 bound to both the Rap1 GTPase and the Heart of Glass (HEG1) cytoplasmic tail

Released:
DOI: 10.2210/pdb4hdq/pdb
PDB entry 4hdq coloured by chain, front view.
PDB entry 4hdq coloured by chain, side view.
PDB entry 4hdq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the ternary complex of Krev interaction trapped 1 (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1) cytoplasmic tail.
Gingras AR, Puzon-McLaughlin W, Ginsberg MH
J Biol Chem 288 23639-49 (2013)
PMID: 23814056

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-126335 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Krev interaction trapped protein 1 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00522 (Residues: 417-736; Coverage: 44%)
Gene names: CCM1, KRIT1
Sequence domains: FERM central domain
Structure domains:
Ras-related protein Rap-1b Chain: B
Molecule details ›
Chain: B
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61224 (Residues: 1-167; Coverage: 91%)
Gene names: OK/SW-cl.11, RAP1B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Protein HEG homolog 1 Chain: C
Molecule details ›
Chain: C
Length: 26 amino acids
Theoretical weight: 3.17 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9ULI3 (Residues: 1356-1381; Coverage: 2%)
Gene names: HEG1, KIAA1237

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 57.31Å b: 77.41Å c: 58.57Å
α: 90° β: 95.56° γ: 90°
R-values:
R R work R free
0.203 0.2 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

Cerebral cavernous malformation proteins at a glance.
Draheim et al. (2014)
10 more

4 mentions without citation

The structure of the ternary complex of Krev interaction trapped 1 (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1) cytoplasmic tail.
Gingras et al. (2013)
3 more