› 4hds

X-ray diffraction
2.4Å resolution

Crystal Structure of ArsAB in Complex with Phenol.

Released:
DOI: 10.2210/pdb4hds/pdb
PDB entry 4hds coloured by chain, front view.
PDB entry 4hds coloured by chain, side view.
PDB entry 4hds coloured by chain, top view.
Source organism: Sporomusa ovata
Primary publication:
Structural insights into the function of the nicotinate mononucleotide:phenol/p-cresol phosphoribosyltransferase (ArsAB) enzyme from Sporomusa ovata.
Newmister SA, Chan CH, Escalante-Semerena JC, Rayment I
Biochemistry 51 8571-82 (2012)
PMID: 23039029

Function and Biology Details

Reaction catalysed: 
Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123629 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 36.32 KDa
Source organism: Sporomusa ovata
Expression system: Escherichia coli
UniProt:
  • Canonical: F6MZ55 (Residues: 5-350; Coverage: 99%)
Gene name: arsA
Sequence domains: Phosphoribosyltransferase
Structure domains:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 36.22 KDa
Source organism: Sporomusa ovata
Expression system: Escherichia coli
UniProt:
  • Canonical: F6MZ56 (Residues: 2-350; Coverage: 100%)
Gene names: SpAn4DRAFT_1783, arsB
Sequence domains: Phosphoribosyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand IPH 1 x IPH
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 52.547Å b: 77.213Å c: 151.522Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.241
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Decoding molecular interactions in microbial communities.
Abreu et al. (2016)
7 other articles

1 mention without citation

Structural insights into the function of the nicotinate mononucleotide:phenol/p-cresol phosphoribosyltransferase (ArsAB) enzyme from Sporomusa ovata.
Newmister et al. (2012)