› 4he4

X-ray diffraction
2.05Å resolution

Crystal structure of the yellow fluorescent protein phiYFP (Phialidium sp.)

Released:
DOI: 10.2210/pdb4he4/pdb
PDB entry 4he4 coloured by chain, front view.
PDB entry 4he4 coloured by chain, side view.
PDB entry 4he4 coloured by chain, top view.
Source organism: Phialidium sp. SL-2003
Primary publication:
Yellow fluorescent protein phiYFPv (Phialidium): structure and structure-based mutagenesis.
Pletneva NV, Pletnev VZ, Souslova E, Chudakov DM, Lukyanov S, Martynov VI, Arhipova S, Artemyev I, Wlodawer A, Dauter Z, Pletnev S
Acta Crystallogr D Biol Crystallogr 69 1005-12 (2013)
PMID: 23695245

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Yellow fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 27.11 KDa
Source organism: Phialidium sp. SL-2003
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6RYS7 (Residues: 2-233; Coverage: 99%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R32
Unit cell:
a: 102.87Å b: 102.87Å c: 242.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.256
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Orange fluorescent proteins: structural studies of LSSmOrange, PSmOrange and PSmOrange2.
Pletnev et al. (2014)
1 more

10 mentions without citation

The design and evolution of fluorescent protein-based sensors for monoatomic ions in biology.
Baek et al. (2021)
9 more