4he5

X-ray diffraction
1.15Å resolution

Crystal structure of the selenomethionine variant of the C-terminal domain of Geobacillus thermoleovorans putative U32 peptidase

Released:
Primary publication:
Ultratight crystal packing of a 10 kDa protein.
Acta Crystallogr D Biol Crystallogr 69 464-70 (2013)
PMID: 23519421

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-124627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase family U32 C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.5 KDa
Source organism: Geobacillus thermoleovorans CCB_US3_UF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G8N3E1 (Residues: 336-422; Coverage: 21%)
Gene name: GTCCBUS3UF5_28720
Sequence domains: Peptidase family U32 C-terminal domain
Structure domains: Translation factors

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 45.55Å b: 45.55Å c: 60.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.141 0.141 0.17
Expression system: Escherichia coli BL21(DE3)