› 4hff

X-ray diffraction
2.4Å resolution

Crystal structure of the type VI effector-immunity complex Tae4-Tai4 from Salmonella Typhimurium

Released:
DOI: 10.2210/pdb4hff/pdb
PDB entry 4hff coloured by chain, front view.
PDB entry 4hff coloured by chain, side view.
PDB entry 4hff coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein.
Zhang H, Zhang H, Gao ZQ, Wang WJ, Liu GF, Xu JH, Su XD, Dong YH
J Biol Chem 288 5928-39 (2013)
PMID: 23288853

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-187385 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytoplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.6 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93IS4 (Residues: 1-161; Coverage: 100%)
Gene name: STM0277
Sequence domains: Type VI secretion system (T6SS), amidase effector protein 4
Structure domains: endopeptidase fold (from Nostoc punctiforme)
Periplasmic protein Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 12.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZRL5 (Residues: 25-127; Coverage: 81%)
Gene name: STM0278
Sequence domains: Type VI secretion system (T6SS), amidase immunity protein
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P6122
Unit cell:
a: 63.903Å b: 63.903Å c: 365.916Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Type VI secretion system effectors: poisons with a purpose.
Russell et al. (2014)
7 more

5 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
4 more