› 4hfk

X-ray diffraction
2.1Å resolution

Crystal structure of the type VI effector-immunity complex Tae4-Tai4 from Enterobacter cloacae

Released:
DOI: 10.2210/pdb4hfk/pdb
PDB entry 4hfk coloured by chain, front view.
PDB entry 4hfk coloured by chain, side view.
PDB entry 4hfk coloured by chain, top view.
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Primary publication:
Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein.
Zhang H, Zhang H, Gao ZQ, Wang WJ, Liu GF, Xu JH, Su XD, Dong YH
J Biol Chem 288 5928-39 (2013)
PMID: 23288853

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-101837 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative cytoplasmic protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 176 amino acids
Theoretical weight: 19.17 KDa
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3CIJ2 (Residues: 1-163; Coverage: 100%)
Gene name: ECL_01542
Sequence domains: Type VI secretion system (T6SS), amidase effector protein 4
Structure domains: endopeptidase fold (from Nostoc punctiforme)
Putative uncharacterized protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 105 amino acids
Theoretical weight: 11.7 KDa
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3CIX8 (Residues: 19-117; Coverage: 100%)
Gene name: ECL_01543
Sequence domains: Type VI secretion system (T6SS), amidase immunity protein
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: C2
Unit cell:
a: 91.342Å b: 138.144Å c: 64.462Å
α: 90° β: 127.89° γ: 90°
R-values:
R R work R free
0.159 0.157 0.204
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Type VI secretion system effectors: poisons with a purpose.
Russell et al. (2014)
8 more

4 mentions without citation

Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein.
Zhang et al. (2013)
3 more