› 4hfl

X-ray diffraction
2Å resolution

Crystal structure of the type VI effector Tae4 from Enterobacter cloacae

Released:
DOI: 10.2210/pdb4hfl/pdb
PDB entry 4hfl coloured by chain, front view.
PDB entry 4hfl coloured by chain, side view.
PDB entry 4hfl coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein.
Zhang H, Zhang H, Gao ZQ, Wang WJ, Liu GF, Xu JH, Su XD, Dong YH
J Biol Chem 288 5928-39 (2013)
PMID: 23288853

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-101835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cytoplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 19.17 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3CIJ2 (Residues: 1-163; Coverage: 100%)
Gene name: ECL_01542
Sequence domains: Type VI secretion system (T6SS), amidase effector protein 4
Structure domains: endopeptidase fold (from Nostoc punctiforme)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P3221
Unit cell:
a: 84.283Å b: 84.283Å c: 43.452Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.251
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Type VI secretion system effectors: poisons with a purpose.
Russell et al. (2014)
7 more

5 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
4 more