› 4hfu

X-ray diffraction
3.11Å resolution

Crystal structure of Fab 8M2 in complex with a H2N2 influenza virus hemagglutinin

Released:
DOI: 10.2210/pdb4hfu/pdb
PDB entry 4hfu coloured by chain, front view.
PDB entry 4hfu coloured by chain, side view.
PDB entry 4hfu coloured by chain, top view.
Source organisms:
Primary publication:
A recurring motif for antibody recognition of the receptor-binding site of influenza hemagglutinin.
Xu R, Krause JC, McBride R, Paulson JC, Crowe JE, Wilson IA
Nat Struct Mol Biol 20 363-70 (2013)
PMID: 23396351

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer (preferred)
hetero 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-208954 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.57 KDa
Source organism: Influenza A virus (A/Japan/305+/1957(H2N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03451 (Residues: 15-340; Coverage: 59%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 174 amino acids
Theoretical weight: 20.14 KDa
Source organism: Influenza A virus (A/Japan/305+/1957(H2N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03451 (Residues: 341-514; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Fab 8M2 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 23.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab 8M2 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R32
Unit cell:
a: 129.59Å b: 129.59Å c: 536.885Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.25
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

25 review citations

Continuing challenges in influenza.
Webster et al. (2014)
24 more

12 mentions without citation

Structural characterization of viral epitopes recognized by broadly cross-reactive antibodies.
Lee et al. (2015)
11 more