› 4hgv

X-ray diffraction
2.09Å resolution

Crystal structure of a fumarate hydratase

Released:
DOI: 10.2210/pdb4hgv/pdb
PDB entry 4hgv coloured by chain, front view.
PDB entry 4hgv coloured by chain, side view.
PDB entry 4hgv coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Eswaramoorthy S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 495 amino acids
Theoretical weight: 53.55 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92PB6 (Residues: 1-463; Coverage: 100%)
Gene names: R01859, SMc00149, fumC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 72.163Å b: 159.965Å c: 162.041Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.234
Expression system: Escherichia coli BL21(DE3)

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