› 4hh0

X-ray diffraction
2.6Å resolution

Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides

Released:
DOI: 10.2210/pdb4hh0/pdb
PDB entry 4hh0 coloured by chain, front view.
PDB entry 4hh0 coloured by chain, side view.
PDB entry 4hh0 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.
Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I
Nat Struct Mol Biol 20 859-67 (2013)
PMID: 23728293

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 43.86 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53119 (Residues: 3-399; Coverage: 88%)
Gene name: appA
Sequence domains: Sensors of blue-light using FAD
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMN 2 x FMN
Ligand CL 18 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3212
Unit cell:
a: 70.26Å b: 70.26Å c: 382.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
11 more

5 mentions without citation

Seeing the light with BLUF proteins.
Park et al. (2017)
4 more