› 4hh2

X-ray diffraction
2.8Å resolution

Structure of PpsR without the HTH motif from Rb. sphaeroides

Released:
DOI: 10.2210/pdb4hh2/pdb
PDB entry 4hh2 coloured by chain, front view.
PDB entry 4hh2 coloured by chain, side view.
PDB entry 4hh2 coloured by chain, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.
Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I
Nat Struct Mol Biol 20 859-67 (2013)
PMID: 23728293

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAS domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 384 amino acids
Theoretical weight: 42.15 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3J179 (Residues: 2-379; Coverage: 82%)
Gene names: RSP_0282, ppsR
Sequence domains: PAS domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 117.45Å b: 117.45Å c: 211.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
12 more

8 mentions without citation

PAS domains in bacterial signal transduction.
Stuffle et al. (2021)
7 more