› 4hi4

X-ray diffraction
2.3Å resolution

Crystal structure of the 5-coordinate ferric heme-binding PAS domain of Aer2 from P. aeruginosa

Released:
DOI: 10.2210/pdb4hi4/pdb
PDB entry 4hi4 coloured by chain, front view.
PDB entry 4hi4 coloured by chain, side view.
PDB entry 4hi4 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Architecture of the soluble receptor Aer2 indicates an in-line mechanism for PAS and HAMP domain signaling.
Airola MV, Huh D, Sukomon N, Widom J, Sircar R, Borbat PP, Freed JH, Watts KJ, Crane BR
J Mol Biol 425 886-901 (2013)
PMID: 23274111

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-191472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein McpB Chains: A, B, D, G
Molecule details ›
Chains: A, B, D, G
Length: 121 amino acids
Theoretical weight: 13.37 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I6V6 (Residues: 174-289; Coverage: 17%)
Gene names: PA0176, aer-2, aer2, mcpB, tlpG
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

3 bound ligands:
Ligand HEM 4 x HEM
Ligand CL 1 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 67.365Å b: 67.435Å c: 117.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.248
Expression system: Escherichia coli

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Citations

4 review citations

The effect of bacterial chemotaxis on host infection and pathogenicity.
Matilla et al. (2018)
3 more

13 mentions without citation

Sensory Repertoire of Bacterial Chemoreceptors.
Ortega et al. (2017)
12 more