› 4hik

X-ray diffraction
1.64Å resolution

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTACGGT)

Released:
DOI: 10.2210/pdb4hik/pdb
Model geometry
Fit model/data
PDB entry 4hik coloured by chain, front view.
PDB entry 4hik coloured by chain, side view.
PDB entry 4hik coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Nonspecific recognition is achieved in Pot1pC through the use of multiple binding modes.
Dickey TH, McKercher MA, Wuttke DS
Structure 21 121-132 (2013)
PMID: 23201273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117590 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 17.37 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13988 (Residues: 198-339; Coverage: 26%)
Gene names: SPAC26H5.06, pot1
Sequence domains: ssDNA-binding domain of telomere protection protein
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(*GP*GP*TP*TP*AP*CP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 44.87Å b: 57.39Å c: 66.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Structural biology of telomeres and telomerase.
Smith et al. (2020)
15 other articles

8 mentions without citation

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
7 more