› 4hj4

X-ray diffraction
2.7Å resolution

Crystal Structure of Rhodobacter Sphaeroides LOV protein

Released:
DOI: 10.2210/pdb4hj4/pdb
PDB entry 4hj4 coloured by chain, front view.
PDB entry 4hj4 coloured by chain, side view.
PDB entry 4hj4 coloured by chain, top view.
Source organism: Cereibacter sphaeroides ATCC 17025
Primary publication:
Light-induced subunit dissociation by a light-oxygen-voltage domain photoreceptor from Rhodobacter sphaeroides.
Conrad KS, Bilwes AM, Crane BR
Biochemistry 52 378-91 (2013)
PMID: 23252338

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.53 KDa
Source organism: Cereibacter sphaeroides ATCC 17025
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M1E1G2 (Residues: 1-177; Coverage: 100%)
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 65.8Å b: 70.5Å c: 74.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.194 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
12 more

4 mentions without citation

The clinical potential of optogenetic interrogation of pathogenesis.
Gao et al. (2023)
3 more