4hja

X-ray diffraction
2.1Å resolution

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (ACGGTTACGGT)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-117598 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 17.18 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13988 (Residues: 198-339; Coverage: 26%)
Gene names: SPAC26H5.06, pot1
Sequence domains: ssDNA-binding domain of telomere protection protein
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(*AP*CP*GP*GP*TP*TP*AP*CP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 45.85Å b: 56.776Å c: 66.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.193 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided